Bio::Restriction::EnzyUserlContributed PeBio::Restriction::EnzymeCollection(3)NAMEBio::Restriction::EnzymeCollection - Set of restriction endonucleases
SYNOPSIS
use Bio::Restriction::EnzymeCollection;
# Create a collection with the default enzymes.
my $default_collection = Bio::Restriction::EnzymeCollection->new();
# Or create a collection from a REBASE 'withrefm' file obtained from
# ftp://ftp.neb.com/pub/rebase/. (See Bio::Restriction::IO for more
# information.)
my $rebase = Bio::Restriction::IO->new(
-file => 'withrefm.610',
-format => 'withrefm' );
my $rebase_collection = $rebase->read();
# Or create an empty collection and set the enzymes later. See
# 'CUSTOM COLLECTIONS' below for more information.
my $empty_collection =
Bio::Restriction::EnzymeCollection->new( -empty => 1 );
# Get an array of Bio::Restriction::Enzyme objects from the collection.
my @enzymes = $default_collection->each_enzyme();
# Get a Bio::Restriction::Enzyme object for a particular enzyme by name.
my $enz = $default_collection->get_enzyme( 'EcoRI' );
# Get a Bio::Restriction::EnzymeCollection object containing the enzymes
# that have the equivalent of 6-bp recognition sequences.
my $six_cutters = $default_collection->cutters( 6 );
# Get a Bio::Restriction::EnzymeCollection object containing the enzymes
# that are rare cutters.
my $rare_cutters = $default_collection->cutters( -start => 6, -end => 8 );
# Get a Bio::Restriction::EnzymeCollection object that contains enzymes
# that generate blunt ends:
my $blunt_cutters = $default_collection->blunt_enzymes();
# See 'CUSTOM COLLECTIONS' below for an example of creating a
# Bio::Restriction::EnzymeCollection object with a specified subset of
# enzymes using methods provided by the Bio::RestrictionEnzyme class.
DESCRIPTIONBio::Restriction::EnzymeCollection represents a collection of
restriction enzymes.
If you create a new collection directly rather than from a REBASE file
using Bio::Restriction::IO, it will be populated by a default set of
enzymes with site and cut information only.
Use Bio::Restriction::Analysis to figure out which enzymes are
available and where they cut your sequence.
CUSTOM COLLECTIONS
Note that the underlying Bio::Restriction::Enzyme objects have a rich
variety of methods that allow more complicated selections than the
methods that are defined by Bio::Restriction::EnzymeCollection.
For example, the way to create a custom collection of Type II enzymes
is as follows:
my $complete_collection =
Bio::Restriction::EnzymeCollection->new();
my $type_ii_collection =
Bio::Restriction::EnzymeCollection->new( -empty => 1 );
$type_ii_collection->enzymes(
grep { $_->type() eq 'II' } $complete_collection->each_enzyme() );
SEE ALSO
Bio::Restriction::IO - read in enzymes from REBASE files
Bio::Restriction::Analysis - figure out what enzymes cut a sequence
Bio::Restriction::Enzyme - define a single restriction enzyme
FEEDBACK
Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
Support
Please direct usage questions or support issues to the mailing list:
bioperl-l@bioperl.org
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly address
it. Please include a thorough description of the problem with code and
data examples if at all possible.
Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via the
web:
http://bugzilla.open-bio.org/
AUTHOR
Rob Edwards, redwards@utmem.edu
CONTRIBUTORS
Heikki Lehvaslaiho, heikki-at-bioperl-dot-org
COPYRIGHT
Copyright (c) 2003 Rob Edwards.
Some of this work is Copyright (c) 1997-2002 Steve A. Chervitz. All
Rights Reserved.
This module is free software; you can redistribute it and/or modify it
under the same terms as Perl itself.
APPENDIX
Methods beginning with a leading underscore are considered private and
are intended for internal use by this module. They are not considered
part of the public interface and are described here for documentation
purposes only.
new
Title : new
Function : Initializes the Restriction::EnzymeCollection object
Returns : The Restriction::EnzymeCollection object
Arguments : optional named parameter -empty
Set parameter -empty to true if you do NOT want the collection be
populated by the default set of prototype type II enzymes.
Alternatively, pass an array of enzymes to -enzymes parameter.
Manipulate the enzymes within the collection
enzymes
Title : enzyme
Function : add/get method for enzymes and enzyme collections
Returns : object itself
Arguments : array of Bio::Restriction::Enzyme and
Bio::Restriction::EnzymeCollection objects
each_enzyme
Title : each_enzyme
Function : get an array of enzymes
Returns : array of Bio::Restriction::Enzyme objects
Arguments : -
get_enzyme
Title : get_enzyme
Function : Gets a Bio::Restriction::Enzyme object for the enzyme name
Returns : A Bio::Restriction::Enzyme object or undef
Arguments : An enzyme name that is in the collection
available_list
Title : available_list
Function : Gets a list of all the enzymes that we know about
Returns : A reference to an array with all the enzyme names
that we have defined or 0 if none are defined
Arguments : Nothing
Comments : Note, I maintain this for backwards compatibility,
but I don't like the name as it is very ambiguous
longest_cutter
Title : longest_cutter
Function : Gets the enzyme with the longest recognition site
Returns : A Bio::Restriction::Enzyme object
Arguments : Nothing
Comments : Note, this is used by Bio::Restriction::Analysis
to figure out what to do with circular sequences
Filter enzymes
blunt_enzymes
Title : blunt_enzymes
Function : Gets a list of all the enzymes that are blunt cutters
Returns : A reference to an array with all the enzyme names that
are blunt cutters or 0 if none are defined
Arguments : Nothing
Comments :
This is an example of the kind of filtering better done by the scripts
using the rich collection of methods in Bio::Restriction::Enzyme.
cutters
Title : cutters
Function : Gets a list of all the enzymes that recognize a
certain size, e.g. 6-cutters
Usage : $cutters = $collection->cutters(6);
Returns : A reference to an array with all the enzyme names
that are x cutters or 0 if none are defined
Arguments : A positive number for the size of cutters to return
OR
A range: (-start => 6, -end => 8,
-inclusive => 1, -exclusive = 0 )
The default for a range is 'inclusive'
perl v5.14.12011-07Bio::Restriction::EnzymeCollection(3)
