Bio::Tools::Run::RNAMoUser3Contributed Perl DocumeBio::Tools::Run::RNAMotif(3)NAMEBio::Tools::Run::RNAMotif - Wrapper for local execution of rnamotif,
rm2ct, rmfmt, rmprune
SYNOPSIS
#run rnamotif|rmfmt|rm2ct
my @params = (
descr => 'pyrR.descr',
fmt => 'gb',
setvar => 'ctx_maxlen=20',
context => 1,
sh => 1,
);
my $factory = Bio::Tools::Run::RNAMotif->new(-program =>'rnamotif',
-prune => 1,
@params);
# Pass the factory a Bio::Seq object or a file name
# Returns a Bio::SearchIO object
#my $searchio = $factory->run("B_sub.gb");
my $searchio = $factory->run($seq);
while (my $result = $searchio->next_result){
while(my $hit = $result->next_hit){
while (my $hsp = $hit->next_hsp){
print join("\t", ( $r->query_name,
$hit->name,
$hsp->hit->start,
$hsp->hit->end,
$hsp->meta,
$hsp->score,
)), "\n";
}
}
}
# Pass a finished report through rmfmt (-a format only)
# Returns Bio::AlignIO object
my $aio = Bio::AlignIO->new(-file=>"rna.msf",-format=>'msf');
my $factory = Bio::Tools::Run::RNAMotif->new('program'=>'rmfmt',
'a' => 1);
my $alnin = $factory->run('trna.rnamotif');
my $aln = $alnin->next_aln;
$aio->write_aln($aln);
DESCRIPTION
Wrapper module for Tom Macke and David Cases's RNAMotif suite of
programs. This allows running of rnamotif, rmprune, rm2ct, and rmfmt.
Binaries are available at
http://www.scripps.edu/mb/case/casegr-sh-3.5.html.
This wrapper allows for one to save output to an optional named file or
tempfile using the '-outfile_name' or '-tempfile' parameters; this is
primarily for saving output from the rm2ct program, which currently
does not have a parser available. If both a named output file and
tempfile flag are set, the output file name is used. The default
setting is piping output into a filehandle for parsing (or output to
STDERR, for rm2ct which requires '-verbose' set to 1).
WARNING: At this time, there is very little checking of parameter
settings, so one could have an error if setting the worng parameter for
a program. Future versions will likely add some error checking.
NOTES ON PROGRAM PARAMETERS
All program parameters are currently supported. Of note, the 'D'
parameter, used for setting the value of a variable to a value, is
changed to 'set_var' to avoid name collisions with 'd' (used for
dumping internal data structures).
FEEDBACK
Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
Support
Please direct usage questions or support issues to the mailing list:
bioperl-l@bioperl.org
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly address
it. Please include a thorough description of the problem with code and
data examples if at all possible.
Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via the
web:
http://bugzilla.open-bio.org/
AUTHOR - Chris Fields
Email: cjfields-at-uiuc-dot-edu
CONTRIBUTORS
cjfields-at-uiuc-dot-edu
APPENDIX
The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _
new
Title : new
Usage : my $wrapper = Bio::Tools::Run::RNAMotif->new(@params)
Function: creates a new RNAMotif factory
Returns: Bio::Tools::Run::RNAMotif
Args : list of parameters
-tempfile => set tempfile flag (default 0)
-outfile_name => set file to send output to (default none)
-prune => set rmprune postprocess flag (default 0)
program_name
Title : program_name
Usage : $factory>program_name()
Function: holds the program name
Returns: string
Args : None
program_dir
Title : program_dir
Usage : $factory->program_dir(@params)
Function: returns the program directory, obtained from ENV variable.
Returns: string
Args :
version
Title : version
Usage : $v = $prog->version();
Function: Determine the version number of the program
Example :
Returns : float or undef
Args : none
run
Title : run
Usage : $obj->run($seqFile)
Function: Runs RNAMotif programs, returns Bio::SearchIO/Bio::AlignIO
Returns : Depends on program:
'rnamotif' - returns Bio::SearchIO
'rmfmt -a' - returns Bio::AlignIO
all others - sends output to outfile, tempfile, STDERR
Use search() (for Bio::SearchIO stream) or get_AlignIO() (for
Bio::AlignIO stream) for a uniform Bioperl object interface.
Args : A Bio::PrimarySeqI or file name
Note : This runs any RNAMotif program set via program()
search
Title : search
Usage : $searchio = $obj->search($seqFile)
Function: Runs 'rnamotif' on seqs, returns Bio::SearchIO
Returns : A Bio::SearchIO
Args : A Bio::PrimarySeqI or file name
Note : Runs 'rnamotif' only, regardless of program setting; all other
parameters loaded
get_AlignIO
Title : get_AlignIO
Usage : $aln = $obj->get_AlignIO($seqFile)
Function: Runs 'rmfmt -a' on file, returns Bio::AlignIO
Returns : A Bio::AlignIO
Args : File name
Note : Runs 'rmfmt -a' only, regardless of program setting; only file
name and outfile (if any) are set
tempfile
Title : tempfile
Usage : $obj->tempfile(1)
Function: Set tempfile flag. When set, writes output to a tempfile; this
is overridden by outfile_name() if set
Returns : Boolean setting (or undef if not set)
Args : [OPTIONAL] Boolean
prune
Title : prune
Usage : $obj->prune(1)
Function: Set rmprune flag. When set, follows any searches with a call to
rmprune (this deletes some redundant sequence hits)
Returns : Boolean setting (or undef if not set)
Args : [OPTIONAL] Boolean
_run
Title : _run
Usage : $obj->_run()
Function: Internal(not to be used directly)
Returns :
Args :
_setparams
Title : _setparams
Usage : Internal function, not to be called directly
Function: creates a string of params to be used in the command string
Example :
Returns : string of params
Args :
_writeSeqFile
Title : _writeSeqFile
Usage : obj->_writeSeqFile($seq)
Function: Internal(not to be used directly)
Returns : writes passed Seq objects to tempfile, to be used as input
for program
Args :
perl v5.14.1 2011-07-21 Bio::Tools::Run::RNAMotif(3)
