Bio::Tools::Run::PhyloUsertContributed)Bio::Tools::Run::Phylo::Njtree::Best(3)NAMEBio::Tools::Run::Phylo::Njtree::Best - Wrapper around the Njtree
(Njtree/phyml) best program.
SYNOPSIS
use Bio::Tools::Run::Phylo::Njtree::Best;
use Bio::AlignIO;
use Bio::TreeIO;
my $alignio = Bio::AlignIO->new(-format => 'fasta',
-file => 't/data/njtree_aln2.nucl.mfa');
my $aln = $alignio->next_aln;
my $treeio = Bio::TreeIO->new(
-format => 'nhx', -file => 't/data/species_tree_njtree.nh');
my $tree = $treeio->next_tree;
my $njtree_best = Bio::Tools::Run::Phylo::Njtree::Best->new();
$njtree_best->alignment($aln);
$njtree_best->tree($tree);
my $nhx_tree = $njtree_best->run();
DESCRIPTION
This is a wrapper around the best program of Njtree by Li Heng. See
http://treesoft.sourceforge.net/njtree.shtml for more information.
Wrapper for the calculation of a reconciled phylogenetic tree with
inferred duplication tags from amultiple sequence alignment and a
species tree using NJTREE.
Helping the module find your executable
You will need to enable NJTREEDIR to find the njtree program. This can
be done in (at least) three ways:
1. Make sure the njtree executable is in your path (i.e.
'which njtree' returns a valid program
2. define an environmental variable NJTREEDIR which points to a
directory containing the 'njtree' app:
In bash
export NJTREEDIR=/home/progs/treesoft/njtree or
In csh/tcsh
setenv NJTREEDIR /home/progs/treesoft/njtree
3. include a definition of an environmental variable NJTREEDIR
in every script that will
BEGIN {$ENV{NJTREEDIR} = '/home/progs/treesoft/njtree'; }
use Bio::Tools::Run::Phylo::Njtree::Best;
FEEDBACK
Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
Support
Please direct usage questions or support issues to the mailing list:
bioperl-l@bioperl.org
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly address
it. Please include a thorough description of the problem with code and
data examples if at all possible.
Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via the
web:
http://bugzilla.open-bio.org/
AUTHOR - Albert Vilella
Email avilella-at-gmail-dot-com
APPENDIX
The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _
program_name
Title : program_name
Usage : $factory->program_name()
Function: holds the program name
Returns: string
Args : None
program_dir
Title : program_dir
Usage : $factory->program_dir(@params)
Function: returns the program directory, obtained from ENV variable.
Returns: string
Args :
new
Title : new
Usage : my $njtree_best = Bio::Tools::Run::Phylo::Njtree::Best->new();
Function: Builds a new Bio::Tools::Run::Phylo::Njtree::Best
Returns : Bio::Tools::Run::Phylo::Njtree::Best
Args : -alignment => the Bio::Align::AlignI object
-tree => the Bio::Tree::TreeI object
-save_tempfiles => boolean to save the generated tempfiles and
NOT cleanup after onesself (default FALSE)
-executable => where the njtree executable resides
See also: Bio::Tree::TreeI, Bio::Align::AlignI
prepare
Title : prepare
Usage : my $rundir = $njtree_best->prepare();
Function: prepare the njtree_best analysis using the default or updated parameters
the alignment parameter and species tree must have been set
Returns : value of rundir
Args : L<Bio::Align::AlignI> object,
L<Bio::Tree::TreeI> object [optional]
run
Title : run
Usage : my $nhx_tree = $njtree_best->run();
Function: run the njtree_best analysis using the default or updated parameters
the alignment parameter must have been set
Returns : L<Bio::Tree::TreeI> object [optional]
Args : L<Bio::Align::AlignI> object
L<Bio::Tree::TreeI> object
error_string
Title : error_string
Usage : $obj->error_string($newval)
Function: Where the output from the last analysus run is stored.
Returns : value of error_string
Args : newvalue (optional)
version
Title : version
Usage : exit if $prog->version() < 1.8
Function: Determine the version number of the program
Example :
Returns : float or undef
Args : none
alignment
Title : alignment
Usage : $njtree_best->align($aln);
Function: Get/Set the L<Bio::Align::AlignI> object
Returns : L<Bio::Align::AlignI> object
Args : [optional] L<Bio::Align::AlignI>
Comment : We could potentially add support for running directly on a file
but we shall keep it simple
See also: L<Bio::SimpleAlign>
tree
Title : tree
Usage : $njtree_best->tree($tree, %params);
Function: Get/Set the L<Bio::Tree::TreeI> object
Returns : L<Bio::Tree::TreeI>
Args : [optional] $tree => L<Bio::Tree::TreeI>,
[optional] %parameters => hash of tree-specific parameters
Comment : We could potentially add support for running directly on a file
but we shall keep it simple
See also: L<Bio::Tree::Tree>
check_names
Title : check_names
Usage :
Function:
Example :
Returns :
Args :
_setparams
Title : _setparams
Usage : Internal function, not to be called directly
Function: Create parameter inputs for njtree_best program
Example :
Returns : parameter string to be passed to njtree_best
during align or profile_align
Args : name of calling object
Bio::Tools::Run::BaseWrapper methods
save_tempfiles
Title : save_tempfiles
Usage : $obj->save_tempfiles($newval)
Function:
Returns : value of save_tempfiles
Args : newvalue (optional)
outfile_name
Title : outfile_name
Usage : my $outfile = $njtree_best->outfile_name();
Function: Get/Set the name of the output file for this run
(if you wanted to do something special)
Returns : string
Args : [optional] string to set value to
tempdir
Title : tempdir
Usage : my $tmpdir = $self->tempdir();
Function: Retrieve a temporary directory name (which is created)
Returns : string which is the name of the temporary directory
Args : none
cleanup
Title : cleanup
Usage : $njtree_best->cleanup();
Function: Will cleanup the tempdir directory
Returns : none
Args : none
io
Title : io
Usage : $obj->io($newval)
Function: Gets a L<Bio::Root::IO> object
Returns : L<Bio::Root::IO>
Args : none
perl v5.14.12011-Bio::Tools::Run::Phylo::Njtree::Best(3)
