Bio::Tools::Phylo::PAMUser Contributed Perl DocumentBio::Tools::Phylo::PAML(3)NAMEBio::Tools::Phylo::PAML - Parses output from the PAML programs codeml,
baseml, basemlg, codemlsites and yn00
SYNOPSIS
#!/usr/bin/perl -Tw
use strict;
use Bio::Tools::Phylo::PAML;
# need to specify the output file name (or a fh) (defaults to
# -file => "codeml.mlc"); also, optionally, the directory in which
# the other result files (rst, 2ML.dS, etc) may be found (defaults
# to "./")
my $parser = Bio::Tools::Phylo::PAML->new
(-file => "./results/mlc", -dir => "./results/");
# get the first/next result; a Bio::Tools::Phylo::PAML::Result object,
# which isa Bio::SeqAnalysisResultI object.
my $result = $parser->next_result();
# get the sequences used in the analysis; returns Bio::PrimarySeq
# objects (OTU = Operational Taxonomic Unit).
my @otus = $result->get_seqs();
# codon summary: codon usage of each sequence [ arrayref of {
# hashref of counts for each codon } for each sequence and the
# overall sum ], and positional nucleotide distribution [ arrayref
# of { hashref of frequencies for each nucleotide } for each
# sequence and overall frequencies ]:
my ($codonusage, $ntdist) = $result->get_codon_summary();
# example manipulations of $codonusage and $ntdist:
printf "There were %d %s codons in the first seq (%s)\n",
$codonusage->[0]->{AAA}, 'AAA', $otus[0]->id();
printf "There were %d %s codons used in all the sequences\n",
$codonusage->[$#{$codonusage}]->{AAA}, 'AAA';
printf "Nucleotide %c was present %g of the time in seq %s\n",
'A', $ntdist->[1]->{A}, $otus[1]->id();
# get Nei & Gojobori dN/dS matrix:
my $NGmatrix = $result->get_NGmatrix();
# get ML-estimated dN/dS matrix, if calculated; this corresponds to
# the runmode = -2, pairwise comparison usage of codeml
my $MLmatrix = $result->get_MLmatrix();
# These matrices are length(@otu) x length(@otu) "strict lower
# triangle" 2D-matrices, which means that the diagonal and
# everything above it is undefined. Each of the defined cells is a
# hashref of estimates for "dN", "dS", "omega" (dN/dS ratio), "t",
# "S" and "N". If a ML matrix, "lnL" and "kappa" will also be defined.
printf "The omega ratio for sequences %s vs %s was: %g\n",
$otus[0]->id, $otus[1]->id, $MLmatrix->[0]->[1]->{omega};
# with a little work, these matrices could also be passed to
# Bio::Tools::Run::Phylip::Neighbor, or other similar tree-building
# method that accepts a matrix of "distances" (using the LOWTRI
# option):
my $distmat = [ map { [ map { $$_{omega} } @$_ ] } @$MLmatrix ];
# for runmode's other than -2, get tree topology with estimated
# branch lengths; returns a Bio::Tree::TreeI-based tree object with
# added PAML parameters at each node
my ($tree) = $result->get_trees();
for my $node ($tree->get_nodes()) {
# inspect the tree: the "t" (time) parameter is available via
# $node->branch_length(); all other branch-specific parameters
# ("omega", "dN", etc.) are available via
# ($omega) = $node->get_tag_values('omega');
}
# if you are using model based Codeml then trees are stored in each
# modelresult object
for my $modelresult ( $result->get_NSSite_results ) {
# model M0, M1, etc
print "model is ", $modelresult->model_num, "\n";
my ($tree) = $modelresult->get_trees();
for my $node ($tree->get_nodes()) {
# inspect the tree: the "t" (time) parameter is available via
# $node->branch_length(); all other branch-specific parameters
# ("omega", "dN", etc.) are available via
# ($omega) = $node->get_tag_values('omega');
}
}
# get any general model parameters: kappa (the
# transition/transversion ratio), NSsites model parameters ("p0",
# "p1", "w0", "w1", etc.), etc.
my $params = $result->get_model_params();
printf "M1 params: p0 = %g\tp1 = %g\n", $params->{p0}, $params->{p1};
# parse AAML result files
my $aamat = $result->get_AADistMatrix();
my $aaMLmat = $result->get_AAMLDistMatrix();
DESCRIPTION
This module is used to parse the output from the PAML programs codeml,
baseml, basemlg, codemlsites and yn00. You can use the
Bio::Tools::Run::Phylo::PAML::* modules to actually run some of the
PAML programs, but this module is only useful to parse the output.
TO DO
Implement get_posteriors(). For NSsites models, obtain arrayrefs of
posterior probabilities for membership in each class for every
position; probabilities correspond to classes w0, w1, ... etc.
my @probs = $result->get_posteriors();
# find, say, positively selected sites!
if ($params->{w2} > 1) {
for (my $i = 0; $i < @probs ; $i++) {
if ($probs[$i]->[2] > 0.5) {
# assumes model M1: three w's, w0, w1 and w2 (positive selection)
printf "position %d: (%g prob, %g omega, %g mean w)\n",
$i, $probs[$i]->[2], $params->{w2}, $probs[$i]->[3];
}
}
} else { print "No positive selection found!\n"; }
FEEDBACK
Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to the
Bioperl mailing list. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
Support
Please direct usage questions or support issues to the mailing list:
bioperl-l@bioperl.org
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly address
it. Please include a thorough description of the problem with code and
data examples if at all possible.
Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track of
the bugs and their resolution. Bug reports can be submitted via the
web:
http://bugzilla.open-bio.org/
AUTHOR - Jason Stajich, Aaron Mackey
Email jason-at-bioperl.org Email amackey-at-virginia.edu
CONTRIBUTORS
Albert Vilella avilella-AT-gmail-DOT-com Sendu Bala bix@sendu.me.uk
TODO
RST parsing -- done, Avilella contributions bugzilla#1506, added by
jason 1.29
-- still need to parse in joint probability and non-syn
changes
at site table
APPENDIX
The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _
new
Title : new
Usage : my $obj = Bio::Tools::Phylo::PAML->new(%args);
Function: Builds a new Bio::Tools::Phylo::PAML object
Returns : Bio::Tools::Phylo::PAML
Args : Hash of options: -file, -fh, -dir
-file (or -fh) should contain the contents of the PAML
outfile;
-dir is the (optional) name of the directory in
which the PAML program was run (and includes other
PAML-generated files from which we can try to gather data)
Implement Bio::AnalysisParserI interface
next_result
Title : next_result
Usage : $result = $obj->next_result();
Function: Returns the next result available from the input, or
undef if there are no more results.
Example :
Returns : a Bio::Tools::Phylo::PAML::Result object
Args : none
perl v5.14.1 2011-07-22 Bio::Tools::Phylo::PAML(3)
