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Bio::Matrix::PSM::ProtUser3Contributed Perl DocumeBio::Matrix::PSM::ProtPsm(3)

NAME
       Bio::Matrix::PSM::ProtPsm - handle combination of site matricies

SYNOPSIS
	 use Bio::Matrix::PSM::IO;

	 #To get a ProtPsm object from a file use the Psm parser:
	 my $psmIO =  Bio::Matrix::PSM::IO->new(-format=>'psiblast', -file=>$file);

	 # Now go through all entities in the file with next_psm, which
	 # returns a Psm object see Bio::Matrix::PSM::IO for detailed
	 # documentation (matrix predictions or matrix sequence matches or
	 # both):

	 while (my $psm=$psmIO->next_psm) {
	    my %psm_header = $psm->header;
	    my $ic    = $psm_header{IC};
	    my $sites = $psm_header{sites};
	    my $width = $psm_header{width};
	    my $score = $psm_header{e_val};
	    my $IUPAC = $psm->IUPAC;
	    my $instances = $psm->instances;
	    foreach my $instance (@{$instances}) {
	      my $id = $instance->primary_id;
	      #Do something with the id
	    }
	  }

DESCRIPTION
       To handle a combination of site matrices and/or their corresponding
       sequence matches (instances). This object inherits from
       Bio::Matrix::PSM::ProtMatrix, so you can methods from that class. It
       may hold also an array of Bio::Matrix::PSM::InstanceSite object, but
       you will have to retrieve these through
       Bio::Matrix::PSM::ProtPsm->instances method (see below). To some extent
       this is an expanded ProtMatrix object, holding data from analysis that
       also deal with sequence matches of a particular matrix.

   DESIGN ISSUES
       This does not make too much sense to me I am mixing PSM with PSM
       sequence matches Though they are very closely related, I am not
       satisfied by the way this is implemented here.  Heikki suggested
       different objects when one has something like meme But does this mean
       we have to write a different objects for mast, meme, transfac,
       theiresias, etc.?  To me the best way is to return SiteMatrix object +
       arrray of InstanceSite objects and then mast will return undef for
       SiteMatrix and transfac will return undef for InstanceSite. Probably I
       cannot see some other design issues that might arise from such
       approach, but it seems more straightforward.  Hilmar does not like this
       beacause it is an exception from the general BioPerl rules. Should I
       leave this as an option?	 Also the header rightfully belongs the driver
       object, and could be retrieved as hashes.  I do not think it can be
       done any other way, unless we want to create even one more object with
       very unclear content.

FEEDBACK
   Mailing Lists
       User feedback is an integral part of the evolution of this and other
       Bioperl modules. Send your comments and suggestions preferably
	to one of the Bioperl mailing lists.  Your participation is much
       appreciated.

	 bioperl-l@bioperl.org			- General discussion
	 http://bioperl.org/wiki/Mailing_lists	- About the mailing lists

   Support
       Please direct usage questions or support issues to the mailing list:

       bioperl-l@bioperl.org

       rather than to the module maintainer directly. Many experienced and
       reponsive experts will be able look at the problem and quickly address
       it. Please include a thorough description of the problem with code and
       data examples if at all possible.

   Reporting Bugs
       Report bugs to the Bioperl bug tracking system to help us keep track
       the bugs and their resolution. Bug reports can be submitted via the
       web:

	 http://bugzilla.open-bio.org/

AUTHOR - James Thompson
       Email tex@biosysadmin.com

DISCLAIMER
       This software is provided "as is" without warranty of any kind.

SEE ALSO
       ProtMatrix, meme, transfac, psiblast, InstanceSite

APPENDIX
   new
	Title	: new
	Usage	: my $psm = Bio::Matrix::PSM::ProtPsm->new(
		     -pS => [ '0', '33', '0', '16', '1', '12', '11', '25' ],
		     -pF => [ '0', '0', '2', '0', '3', '0', '0', '0' ],
		     -pT => [ '0', '8', '7', '10', '1', '2', '7', '8' ],
		     -pN => [ '0', '0', '2', '13', '0', '36', '1', '4' ],
		     -pK => [ '0', '5', '0', '13', '1', '15', '0', '2' ],
		     -pY => [ '0', '0', '0', '0', '0', '0', '0', '0' ],
		     -pE => [ '0', '41', '1', '12', '0', '0', '0', '15' ],
		     -pV => [ '0', '3', '9', '0', '2', '0', '3', '1' ],
		     -pQ => [ '0', '0', '0', '15', '0', '4', '0', '3' ],
		     -pM => [ '100', '0', '66', '0', '2', '0', '0', '0' ],
		     -pC => [ '0', '0', '0', '0', '0', '0', '0', '0' ],
		     -pL => [ '0', '0', '8', '0', '25', '0', '4', '0' ],
		     -pA => [ '0', '10', '1', '9', '2', '0', '22', '16' ],
		     -pW => [ '0', '0', '0', '0', '0', '0', '0', '0' ],
		     -pP => [ '0', '0', '0', '0', '3', '1', '45', '0' ],
		     -pH => [ '0', '0', '0', '0', '0', '0', '1', '0' ],
		     -pD => [ '0', '0', '1', '7', '2', '2', '0', '22' ],
		     -pR => [ '0', '0', '0', '3', '0', '27', '0', '0' ],
		     -pI => [ '0', '0', '3', '0', '59', '1', '2', '3' ],
		     -pG => [ '0', '0', '0', '1', '0', '0', '4', '1' ],
		     -IC => $ic,
		     -sites => $istes,
		     -width => $width,
		     -e_val => $e_val,
		     -instances => $instances,
		  }

	Function: Creates a new Bio::Matrix::PSM::ProtPsm object
	Throws	:
	Example :
	Returns :  Bio::Matrix::PSM::Psm object
	Args	:  hash

   instances
	Title	: instances
	Usage	:   my @instances=@{$psm->instances};
	Function: Gets/sets the instances (Bio::Matrix::PSM::InstanceSite objects)
	       associated with the Psm object
	Throws	:
	Example :
	Returns :  array reference (Bio::Matrix::PSM::InstanceSite objects)
	Args	:  array reference (Bio::Matrix::PSM::InstanceSite objects)

   header
	Title	: header
	Usage	:  my %header=$psm->header;
	       my $ic=$psm->header('IC');
	Function: Gets the general information, common for most files,
	      dealing with PSM such as information content (IC), score
	      (e-value, etc.), number of sites (sites) and width. This
	      list may expand. The current list should be in
	      @Bio::Matrix::PSM::Psm::HEADER. Returns an epty list if an
	      argument is supplied that is not in
	      @Bio::Matrix::PSM::meme::HEADER.
	Throws	:
	Example :
	Returns :  hash or string
	Args	:  string (IC, e_val...)

   matrix
	Title	:  matrix
	Usage	:  my $matrix = $psm->matrix;
	Function:  Gets/sets the SiteMatrix related information
	Throws	:
	Example :
	Returns :  Bio::Matrix::PSM::SiteMatrix objects
	Args	:  Bio::Matrix::PSM::SiteMatrix objects

perl v5.14.1			  2011-07-22	  Bio::Matrix::PSM::ProtPsm(3)
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