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Bio::Index::Fastq(3)  User Contributed Perl Documentation Bio::Index::Fastq(3)

NAME
       Bio::Index::Fastq - Interface for indexing (multiple) fastq files

SYNOPSIS
	   # Complete code for making an index for several
	   # fastq files
	   use Bio::Index::Fastq;
	   use strict;

	   my $Index_File_Name = shift;
	   my $inx = Bio::Index::Fastq->new(
	       '-filename' => $Index_File_Name,
	       '-write_flag' => 1);
	   $inx->make_index(@ARGV);

	   # Print out several sequences present in the index
	   # in Fastq format
	   use Bio::Index::Fastq;
	   use strict;

	   my $Index_File_Name = shift;
	   my $inx = Bio::Index::Fastq->new('-filename' => $Index_File_Name);
	   my $out = Bio::SeqIO->new('-format' => 'Fastq','-fh' => \*STDOUT);

	   foreach my $id (@ARGV) {
	       my $seq = $inx->fetch($id); # Returns Bio::Seq::Quality object
	       $out->write_seq($seq);
	   }

	   # or, alternatively
	   my $id;
	   my $seq = $inx->get_Seq_by_id($id); #identical to fetch

DESCRIPTION
       Inherits functions for managing dbm files from Bio::Index::Abstract.pm,
       and provides the basic funtionallity for indexing fastq files, and
       retrieving the sequence from them. Note: for best results 'use strict'.

       Bio::Index::Fastq supports the Bio::DB::BioSeqI interface, meaning it
       can be used as a Sequence database for other parts of bioperl

FEED_BACK
   Mailing Lists
       User feedback is an integral part of the evolution of this and other
       Bioperl modules. Send your comments and suggestions preferably to one
       of the Bioperl mailing lists.  Your participation is much appreciated.

	 bioperl-l@bioperl.org			- General discussion
	 http://bioperl.org/wiki/Mailing_lists	- About the mailing lists

   Support
       Please direct usage questions or support issues to the mailing list:

       bioperl-l@bioperl.org

       rather than to the module maintainer directly. Many experienced and
       reponsive experts will be able look at the problem and quickly address
       it. Please include a thorough description of the problem with code and
       data examples if at all possible.

   Reporting Bugs
       Report bugs to the Bioperl bug tracking system to help us keep track
       the bugs and their resolution.  Bug reports can be submitted via the
       web:

	 http://bugzilla.open-bio.org/

AUTHOR - Tony Cox
       Email - avc@sanger.ac.uk

APPENDIX
       The rest of the documentation details each of the object methods.
       Internal methods are usually preceded with a _

   _file_format
	Title	: _file_format
	Function: The file format for this package, which is needed
		  by the SeqIO system when reading the sequence.
	Returns : 'Fastq'

   _index_file
	 Title	 : _index_file
	 Usage	 : $index->_index_file( $file_name, $i )
	 Function: Specialist function to index FASTQ format files.
		   Is provided with a filename and an integer
		   by make_index in its SUPER class.
	 Example :
	 Returns :
	 Args	 :

   id_parser
	 Title	 : id_parser
	 Usage	 : $index->id_parser( CODE )
	 Function: Stores or returns the code used by record_id to
		   parse the ID for record from a string.  Useful
		   for (for instance) specifying a different
		   parser for different flavours of FASTQ file.
		   Returns \&default_id_parser (see below) if not
		   set. If you supply your own id_parser
		   subroutine, then it should expect a fastq
		   description line.  An entry will be added to
		   the index for each string in the list returned.
	 Example : $index->id_parser( \&my_id_parser )
	 Returns : ref to CODE if called without arguments
	 Args	 : CODE

   default_id_parser
	 Title	 : default_id_parser
	 Usage	 : $id = default_id_parser( $header )
	 Function: The default Fastq ID parser for Fastq.pm
		   Returns $1 from applying the regexp /^>\s*(\S+)/
		   to $header.
	 Returns : ID string
	 Args	 : a fastq header line string

perl v5.14.1			  2011-07-22		  Bio::Index::Fastq(3)
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