Bio::DasI(3) User Contributed Perl Documentation Bio::DasI(3)NAMEBio::DasI - DAS-style access to a feature database
SYNOPSIS
# Open up a feature database somehow...
$db = Bio::DasI->new(@args);
@segments = $db->segment(-name => 'NT_29921.4',
-start => 1,
-end => 1000000);
# segments are Bio::Das::SegmentI - compliant objects
# fetch a list of features
@features = $db->features(-type=>['type1','type2','type3']);
# invoke a callback over features
$db->features(-type=>['type1','type2','type3'],
-callback => sub { ... }
);
$stream = $db->get_seq_stream(-type=>['type1','type2','type3']);
while (my $feature = $stream->next_seq) {
# each feature is a Bio::SeqFeatureI-compliant object
}
# get all feature types
@types = $db->types;
# count types
%types = $db->types(-enumerate=>1);
@feature = $db->get_feature_by_name($class=>$name);
@feature = $db->get_feature_by_target($target_name);
@feature = $db->get_feature_by_attribute($att1=>$value1,$att2=>$value2);
$feature = $db->get_feature_by_id($id);
$error = $db->error;
DESCRIPTIONBio::DasI is a simplified alternative interface to sequence annotation
databases used by the distributed annotation system (see Bio::Das). In
this scheme, the genome is represented as a series of features, a
subset of which are named. Named features can be used as reference
points for retrieving "segments" (see Bio::Das::SegmentI), and these
can, in turn, be used as the basis for exploring the genome further.
In addition to a name, each feature has a "class", which is essentially
a namespace qualifier and a "type", which describes what type of
feature it is. Das uses the GO consortium's ontology of feature types,
and so the type is actually an object of class Bio::Das::FeatureTypeI
(see Bio::Das::FeatureTypeI). Bio::DasI provides methods for
interrogating the database for the types it contains and the counts of
each type.
FEEDBACK
Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
bioperl-l@bioperl.org
Support
Please direct usage questions or support issues to the mailing list:
bioperl-l@bioperl.org
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly address
it. Please include a thorough description of the problem with code and
data examples if at all possible.
Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via the
web:
http://bugzilla.open-bio.org/
AUTHOR - Lincoln Stein
Email lstein@cshl.org
APPENDIX
The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _
new
Title : new
Usage : Bio::DasI->new(@args)
Function: Create new Bio::DasI object
Returns : a Bio::DasI object
Args : see below
The new() method creates a new object. The argument list is either a
single argument consisting of a connection string, or the following
list of -name=>value arguments:
Argument Description
-------------------
-dsn Connection string for database
-adaptor Name of an adaptor class to use when connecting
-aggregator Array ref containing list of aggregators
"semantic mappers" to apply to database
-user Authentication username
-pass Authentication password
Implementors of DasI may add other arguments.
types
Title : types
Usage : $db->types(@args)
Function: return list of feature types in database
Returns : a list of Bio::Das::FeatureTypeI objects
Args : see below
This routine returns a list of feature types known to the database. It
is also possible to find out how many times each feature occurs.
Arguments are -option=>value pairs as follows:
-enumerate if true, count the features
The returned value will be a list of Bio::Das::FeatureTypeI objects
(see Bio::Das::FeatureTypeI.
If -enumerate is true, then the function returns a hash (not a hash
reference) in which the keys are the stringified versions of
Bio::Das::FeatureTypeI and the values are the number of times each
feature appears in the database.
parse_types
Title : parse_types
Usage : $db->parse_types(@args)
Function: parses list of types
Returns : an array ref containing ['method','source'] pairs
Args : a list of types in 'method:source' form
Status : internal
This method takes an array of type names in the format "method:source"
and returns an array reference of ['method','source'] pairs. It will
also accept a single argument consisting of an array reference with the
list of type names.
segment
Title : segment
Usage : $db->segment(@args);
Function: create a segment object
Returns : segment object(s)
Args : see below
This method generates a Bio::Das::SegmentI object (see
Bio::Das::SegmentI). The segment can be used to find overlapping
features and the raw sequence.
When making the segment() call, you specify the ID of a sequence
landmark (e.g. an accession number, a clone or contig), and a
positional range relative to the landmark. If no range is specified,
then the entire region spanned by the landmark is used to generate the
segment.
Arguments are -option=>value pairs as follows:
-name ID of the landmark sequence.
-class A namespace qualifier. It is not necessary for the
database to honor namespace qualifiers, but if it
does, this is where the qualifier is indicated.
-version Version number of the landmark. It is not necessary for
the database to honor versions, but if it does, this is
where the version is indicated.
-start Start of the segment relative to landmark. Positions
follow standard 1-based sequence rules. If not specified,
defaults to the beginning of the landmark.
-end End of the segment relative to the landmark. If not specified,
defaults to the end of the landmark.
The return value is a list of Bio::Das::SegmentI objects. If the
method is called in a scalar context and there are no more than one
segments that satisfy the request, then it is allowed to return the
segment. Otherwise, the method must throw a "multiple segment
exception".
features
Title : features
Usage : $db->features(@args)
Function: get all features, possibly filtered by type
Returns : a list of Bio::SeqFeatureI objects
Args : see below
Status : public
This routine will retrieve features in the database regardless of
position. It can be used to return all features, or a subset based on
their type
Arguments are -option=>value pairs as follows:
-types List of feature types to return. Argument is an array
of Bio::Das::FeatureTypeI objects or a set of strings
that can be converted into FeatureTypeI objects.
-callback A callback to invoke on each feature. The subroutine
will be passed each Bio::SeqFeatureI object in turn.
-attributes A hash reference containing attributes to match.
The -attributes argument is a hashref containing one or more attributes
to match against:
-attributes => { Gene => 'abc-1',
Note => 'confirmed' }
Attribute matching is simple exact string matching, and multiple
attributes are ANDed together. See Bio::DB::ConstraintsI for a more
sophisticated take on this.
If one provides a callback, it will be invoked on each feature in turn.
If the callback returns a false value, iteration will be interrupted.
When a callback is provided, the method returns undef.
get_feature_by_name
Title : get_feature_by_name
Usage : $db->get_feature_by_name(-class=>$class,-name=>$name)
Function: fetch features by their name
Returns : a list of Bio::SeqFeatureI objects
Args : the class and name of the desired feature
Status : public
This method can be used to fetch named feature(s) from the database.
The -class and -name arguments have the same meaning as in segment(),
and the method also accepts the following short-cut forms:
1) one argument: the argument is treated as the feature name
2) two arguments: the arguments are treated as the class and name
(note: this uses _rearrange() so the first argument must not
begin with a hyphen or it will be interpreted as a named
argument).
This method may return zero, one, or several Bio::SeqFeatureI objects.
The implementor may allow the name to contain wildcards, in which case
standard C-shell glob semantics are expected.
get_feature_by_target
Title : get_feature_by_target
Usage : $db->get_feature_by_target($class => $name)
Function: fetch features by their similarity target
Returns : a list of Bio::SeqFeatureI objects
Args : the class and name of the desired feature
Status : public
This method can be used to fetch a named feature from the database
based on its similarity hit. The arguments are the same as
get_feature_by_name(). If this is not implemented, the interface
defaults to using get_feature_by_name().
get_feature_by_id
Title : get_feature_by_id
Usage : $db->get_feature_by_target($id)
Function: fetch a feature by its ID
Returns : a Bio::SeqFeatureI objects
Args : the ID of the feature
Status : public
If the database provides unique feature IDs, this can be used to
retrieve a single feature from the database. If not overridden, this
interface calls get_feature_by_name() and returns the first element.
get_feature_by_attribute
Title : get_feature_by_attribute
Usage : $db->get_feature_by_attribute(attribute1=>value1,attribute2=>value2)
Function: fetch features by combinations of attribute values
Returns : a list of Bio::SeqFeatureI objects
Args : the class and name of the desired feature
Status : public
This method can be used to fetch a set of features from the database.
Attributes are a list of name=>value pairs. They will be logically
ANDed together. If an attribute value is an array reference, the list
of values in the array is treated as an alternative set of values to be
ORed together.
search_notes
Title : search_notes
Usage : $db->search_notes($search_term,$max_results)
Function: full-text search on features, ENSEMBL-style
Returns : an array of [$name,$description,$score]
Args : see below
Status : public
This routine performs a full-text search on feature attributes (which
attributes depend on implementation) and returns a list of
[$name,$description,$score], where $name is the feature ID,
$description is a human-readable description such as a locus line, and
$score is the match strength.
Since this is a decidedly non-standard thing to do (but the generic
genome browser uses it), the default method returns an empty list. You
do not have to implement it.
get_seq_stream
Title : get_seq_stream
Usage : $seqio = $db->get_seq_stream(@args)
Function: Performs a query and returns an iterator over it
Returns : a Bio::SeqIO stream capable of returning Bio::SeqFeatureI objects
Args : As in features()
Status : public
This routine takes the same arguments as features(), but returns a
Bio::SeqIO::Stream-compliant object. Use it like this:
$stream = $db->get_seq_stream('exon');
while (my $exon = $stream->next_seq) {
print $exon,"\n";
}
NOTE: In the interface this method is aliased to get_feature_stream(),
as the name is more descriptive.
refclass
Title : refclass
Usage : $class = $db->refclass
Function: returns the default class to use for segment() calls
Returns : a string
Args : none
Status : public
For data sources which use namespaces to distinguish reference sequence
accessions, this returns the default namespace (or "class") to use.
This interface defines a default of "Accession".
perl v5.14.1 2011-07-22 Bio::DasI(3)
